凝集素对于糖链结构具有专一识别性,因此广泛用于蛋白糖修饰研究。本芯片固定了56种凝集素,涵盖了几乎所有的主要凝集素,为糖基化研究提供了有力的工具。
凝集素:56种
一张芯片上的点阵数目:4
点样环境:温度4-8℃,湿度30%~40%
芯片基片:晶芯高分子三维基片,尺寸为75*25 mm
点直径:~120微米
样品重复:每个样品重复3个点;
点间距:X向270微米,Y向300微米(圆心距)
点体积:0.5~1 nl/点
芯片点制后处理:4℃过夜固定后-80℃长期保存
凝集素list下载:Lectin Microarray-list-56
1. 特定蛋白表面糖谱构建及差异检测
2. 活细胞表面糖谱构建及差异检测
3. 血清或细胞裂解液糖谱构建及差异检测
4. 糖生物标志物的发现
参考文献
1. Shu-Min Zhou , et al. Lectin RCA-I specifically binds to metastasis-associated cell surface glycans in triple-negative breast cancer. Breast Cancer Research. (2015):1465-5411. [PubMed]
2. Tao SC et al. (2008). Lectin microarrays identify cell-specific and functionally significant cell surface glycan markers. Glycobiology 18(10):761-9. doi: 10.1093/glycob/cwn063. Epub Jul 14. [PubMed]
3. Sun Y, Cheng L, Gu Y, et al. A Human Lectin Microarray for Sperm Surface Glycosylation Analysis[J]. Molecular & Cellular Proteomics, 2016: mcp. M116. 059311. [PubMed]
4. Nakajima K, et al. Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis,Cancer Med. 2015 Feb;4(2):293-302. [PubMed]
5. Roy B, et al. On-chip lectin microarray for glycoprofiling of different gastritis types and gastric cancer. Biomicrofluidics. 2014 Jun 6;8(3):034107.[PubMed]
6. Wu J , et al. Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC−MS/MS. J Proteome Res. 2012 Sep 7;11(9):4541-52.[PubMed]
7. Li Y, et al. Detection and verification of glycosylation patterns of glycoproteins from clinical specimens using lectin microarrays and lectin-based immunosorbent assays. Anal Chem. 2011 Nov 15;83(22):8509-16.
8. Zhou SM et al. Lectin microarrays: a powerful tool for glycan-based biomarker discovery. Comb Chem High Throughput Screen. 2011 Sep;14(8):711-9.[PubMed]
9. Li Y , et al. High-throughput lectin microarray-based analysis of live cell surface glycosylation. Curr Protoc Protein Sci. 2011 Feb;Chapter 12:Unit12.9.[PubMed]