Microbial gene knockout based on Rec homologous recombination method

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Service Introduction:
The traditional method of bacterial gene knockout is to use the bacteria's own Rec system to perform homologous recombination of the incoming foreign DNA and its own target gene, and perform allelic replacement to achieve gene knockout. By designing a homologous fusion fragment of the target gene, it is transformed together with a suicide vector containing a recombinase, and the fragment is recombined and inserted into the target bacterial genome. Through antibiotic screening, mutant strains with integrated target sites in the bacterial genome were obtained. Under the pressure of the second round of reverse selection, only bacteria that have undergone a second homologous recombination in the bacterial genome and lost the suicide plasmid can survive. After that, the mutants with target gene deletion can be obtained by PCR identification. Gene mutants are powerful tools for studying microbial gene functions and have important scientific research value.

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