Protein in vitro binding experiment

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In vitro protein binding experiment, also known as Pull-down experiment, is to target-GST (Glutathione-S-transferase glutathione thiol transferase) fusion protein affinity solidified on glutathione-labeled magnetic beads as The target protein affinity support acts as a kind of "bait protein". The target protein solution is incubated with it to capture the "capture protein" (target protein) that interacts with it, and the conjugate is eluted by SDS-PAGE electrophoresis Analysis to confirm the interaction between the two proteins or to screen the corresponding target protein. Both "bait protein" and "capture protein" can be obtained by cell lysates, purified proteins, expression systems, and in vitro transcription and translation systems. This method is simple and easy to operate.

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