Western blot analysis

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Western blot analysis, or Western Blot, is a method of transferring proteins to a membrane and then using antibodies for detection. For natural proteins, the corresponding antibody can be used as the primary antibody for detection. For the new gene without primary antibody, the tag with the antibody can be fused by recombinant expression, and the antibody can be detected by the fusion part. This technique is widely used to detect protein expression levels and as a downstream detection method for protein interaction analysis techniques such as immunoprecipitation.
The protein sample separated by PAGE is transferred to a solid phase carrier (such as nitrocellulose membrane). The solid phase carrier adsorbs the protein in the form of non-covalent bonds, and can maintain the type of polypeptide separated by electrophoresis. The protein or peptide on the solid phase carrier is used as an antigen to react with the corresponding antibody, and then react with the enzyme or isotope-labeled secondary antibody, after the substrate color development or autoradiography to detect the specific purpose of electrophoretic separation The protein component of gene expression.

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