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Place the Transwell chamber in the culture plate. The chamber is called the upper chamber, and the culture plate is called the lower chamber. The upper chamber contains the upper culture medium, and the lower chamber contains the lower culture medium. The upper and lower culture medium are separated by a polycarbonate membrane. We planted the cells in the upper chamber. Due to the permeability of the polycarbonate membrane, the components in the lower culture medium can affect the cells in the upper chamber, so that we can study the effects of the components in the lower culture medium on cell growth and movement. Using polycarbonate membranes with different pore sizes and different treatments, you can conduct studies in various areas such as co-culture, cell chemotaxis, cell migration, and cell invasion. Transwell migration experiments commonly use 8.0 and 12.0 µm membranes, tumor cells are grown in the upper chamber, and FBS or some specific chemokines are added to the lower chamber. The tumor cells will run to the lower chamber with high nutritional content. The ability of tumor cells to migrate.
