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  No matter the primary tumor or secondary tumor, once the growth diameter exceeds 1-2 mm, there will be angiogenesis. This is because tumor cells can secrete a variety of growth factors and induce angiogenesis. The angiogenesis of most malignant tumors is dense and grows rapidly. Therefore, angiogenesis plays an important role in the development and metastasis of tumors. Inhibiting this process will obviously prevent the development and spread of tumor tissues.

  In the process of blood vessel formation, endothelial cells form cell lines and then form lumens, which can also form lumens in vitro when cultured under specific conditions such as matrigel and collagen. The drug can inhibit the formation of blood vessels by inhibiting the formation of the lumen or breaking the formed lumen. With the help of computer software, the number of small tubes and the number of connections between the small tubes and the length and area of the small tubes can be calculated to quantitatively analyze the influence of drugs on the formation of the lumen.

  Technical principle: Use Matrigel to simulate the body environment, inoculate tumor cells on it, and observe the angiogenesis.


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